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Rapid determination and pharmacokinetics study of lignans in rat plasma after oral administration of Schisandra chinensis extract and pure deoxyschisandrin

Date:2016/8/29 21:12:22

Rapid determination and pharmacokinetics study of lignans in rat plasma after oral administration of Schisandra 

chinensis extract and pure deoxyschisandrin. Abstract A simple, sensitive and rapid method for analysis of six 

lignans in rat plasma after oral administration of Schisandra chinensis extracts, utilizing liquid chromatography 

tandem mass spectrometry (LC-MS), was established and validated. Plasma samples were prepared by one-step protein 

precipitation using acetonitrile and the analytes were separated on an SB-C6966 column (10065mm65×653.065mm, 3.56508m) 

with the mobile phase of acetonitrile-water at a flow-rate of 0.8 mL/min. 


Analytes were determined in a single-quadrupole mass spectrometer in the selected ion monitoring (SIM) mode using 

electrospray source with positive mode. The method was proved to be rapid, sensitive and reproducible, and it was 

successfully applied to the pharmacokinetic studies of six lignans in rat plasma after oral administration of 

Schisandra chinensis extracts. In this research, the pharmacokinetics of deoxyschisandrin was also studied following 

oral administration of the pure deoxyschisandrin. It was found that most of the pharmacokinetic parameters of 

deoxyschisandrin in the extract were changed significantly compared with those in monomer. The content assay 

also revealed that the concentrations of the lignan in the extract increased in vivo compared with the pure 

monomer. Some ingredients in the extract may increase the dissolution of deoxyschisandrin, delay its elimination 

and enhance its bioavailability in rat. Copyright 08 2010 John Wiley & Sons, Ltd.