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Simultaneous determination of 4 nortriterpenoids in Schisandra chinensis extract by HPLC

Date:2016/8/23 20:42:53

Simultaneous determination of 4 nortriterpenoids in Schisandra chinensis extract by HPLC

To determine 4 nortriterpenoids(de-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A,

lancifodilactone D) in Schisandra chinensis extract by HPLC.Method: The analysis was performed on a waters 

symmetry column(4.6 mm×250 mm,5 μm)with the mobile phase of acetonitrile-water(33∶67) at a flow rate of 

1ml• min-1.The column temperature was set at 37℃,and the detector wavelength was 264 nm.Result: The linear 

ranges of de-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A,and lancifodilactone D are 

0.075-1.800,0.098-0.980;0.095-0.950,and 0.053-0.530 μg,respectively,and the average recoveries were 98.57%,

96.44%,97.96%,and 97.27%,respectively.Conclusion: The four nortriterpenoids were well separated by this method,

and it could be used to determine the four nortriterpenoids in Schisandra chinensis extract.