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In vitro protective effect of Schisandra chinensis extract against carbon tetrachlorideinduced hepatotoxicity in common carp (Cyprinus carpio)

Date:2016/8/22 21:09:52

In vitro protective effect of Schisandra chinensis extract against carbon tetrachlorideinduced hepatotoxicity in

common carp (Cyprinus carpio).In the present study, in vitro hepatoprotective effect of Schisandra chinensis 

extract (SCE) was evaluated against carbon tetrachloride (CCl4)ndash;induced hepatotoxicity in common carp. 

SCE (100, 200, and 400 g ml-1) was added to the carp primary hepatocytes before (pre-treatment), after 

(post-treatment), and both before and after (pre and post-treatment) the exposure of the hepatocytes to 8 mM 

CCl4 in the culture medium. Results showed thatexposure of the primary cultured carp hepatocytes to 8 mM CCl4 

for 4 h caused cytotoxicity, manifested by loss of cell viability and significantly elevated levels of lactate 

dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT) and malondialde

hyde (MDA), and significantly reduced activities of superoxide dismutase (SOD) and glutathione peroxidase 

(GSH-Px) in the supernatant. Pre-treatment and pre and post-treatment of the hepatocytes with SCE significantly 

reduced the elevated levels of LDH, GOT, GPT and MDA; increased the reduhttp://www.world2source.com/Schisandra-Chinensis-Extract.htmlced activities of SOD and GSH-Px and 

increased the cell viability in a dose-dependent manner. Post-treatment of the hepatocytes with SCE did not 

show significant effects on the tested parameters except GPT. The results suggest that SCE is a potent hepatoprotective 

agent that could protect fish hepatocytes against the acute injury and this ability might be attributed to its 

antioxidant potential. The results also imply that SCEcan be potentially used for preventing rather than curing 

liver diseases in fish. Key words: Carp primary hepatocytes, hepatoprotection, antioxidant, Schisandra chinensis,

Schisandra chinensis extract.

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