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Lignans of Schisandra chinensis extract: isolation and analysis in plant cell cultures

Date:2016/10/8 16:03:27

Lignans of Schisandra chinensis extract: isolation and analysis in plant cell cultures. The fruit of 

Schisandra chinensis extract, a woody liana, has been used for centuries in traditional Chinese medicine.

The fruit is prescribed for the treatment of hepatitis in China. Active principles are lignans with unusual 

structures derived from dibenzo[a,c]cyclooctadiene. These lignans have been shown to possess a broad range 

of biological effects. To obtain pure standards of lignans, 349 g of Schisandra chinensis extract were 

extracted with petroleum ether. The fraction rich in lignans was obtained by extraction of petroleum ether 

fraction with methanol. Methanol extract was fractionated on silica gel column and subsequently by high 

performance liquid chromatography on reversed phase columns 250 x 25 and 250 x 8 mm (Tessek SGX C18, Prague)

with methanol and water as a mobile phase. Seven lignans, schizandrin, tigloylgomisin H, gomisin A, deoxyschizandrin, 

gamma-schizandrin, gomisin N and wuweizisu C, were isolated. 

Their structures were identified by spectral methods (1D and 2D NMR, UV, MS) and confirmed with those published 

previously. High performance liquid chromatography coupled with ultraviolet detection was used for the determination 

of lignans in the samples of cell cultures of Schisandra chinensis extract. Methanol was found to be the 

most efficient solvent for the extraction of lignans from freeze-dried samples. Methanolic extracts and media 

samples were cleaned by solid-phase extraction with Strata C18-E (Phenomenex) cartridges. After the loading 

of the samples, the cartridges were washed with 40 % aqueous methanol (v/v) to remove interfering compounds.

The lignans were eluted from the cartridges with methanol and examined by HPLC. The chromatographic separation 

was carried out on Chromolith Performance RP-18e monolith column (100 x 4.6 mm, Merck) using isocratic mobile 

phase of acetonitrile and water in the ratio 50:50 (v/v). The base line separation of lignans was achieved 

within a short time (20 minutes) owing to use of the monolithic column, which enables the high flow rate of

mobile phase (2 ml/min).