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Extraction and Antibacterial Activity of Polysaccharides from Schisandra chinensis extract(Turcz.) Baill Leaves

Date:2016/10/8 15:59:16

Extraction and Antibacterial Activity of Polysaccharides from Schisandra chinensis extract(Turcz.) Baill Leaves.The extraction of polysaccharides from Schisandra chinensis extract(Turcz.) Baill leaves(PSL) was optimized using onefactor-at-a-time and orthogonal array design method.Meanwhile,the inhibitory activities of PSL against Staphylococcus aureus,Escherichia coli and Bacillus subtilis were investigated.


The results showed that the optimal conditions for PSL extraction were determined as two extraction cycles with distilled water at a solid/solvent ratio of 1:45(g/mL) at 90 for 1 h.Under these conditions,the extraction yield of PSL was 9.08%.The PSL obtained could not be effectively deproteinized by Sevag method according to UV spectroscopic analysis and its color was darkened.Meanwhile,IR spectroscopic analysis demonstrated that this PSL possessed common spectral characteristics of polysaccharides and peptide bonds,suggesting that it might be formed by conjugation of polysaccharides with proteins or polypeptides through peptide bonds.Furthermore,the polysaccharide extract possessed inhibitory activities against three bacterial strains under investigation.