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Analysis of Drug-induced Constituents in the Serum of Rat After Oral Administration of Schisandra Chinensis extract by HPLC-DAD

Date:2016/9/27 21:46:01

Analysis of Drug-induced Constituents in the Serum of Rat After Oral Administration of Schisandra Chinensis extract by HPLC-DAD.

Objective To analyze drug-induced constituents in serum containing drug(SCD) of Schisandra Chinensis extract.


Methods SCD were prepared after rats of gavage administration of ethanol extract of Schisandra Chinensis extract.Chromatogram fingerprint of SCD,ethanol Schisandra Chinensis extract,reference substance and blank serum were detected by HPLC-DAD and spectrograms of chromatographic peaks of these chromatograms were extracted,respectively.By comparing the retention time and spectrograms of chromatographic peaks,drug-induced constituents in rats serum were analyzed.


Results Thirteen drug-induced constituents were detected in rats after oral administration of Schisandra Chinensis extract,eight of them were the original compounds contained in materia medica and five were metabolites,and four constituents out of eight original form ones were identified as schisandrin,schisandrol B,deoxyschisandrin,and schisandrin B.All the spectrograms of thirteen drug-induced constituents possessed the same character as dibenzocyclooctadiene-like products.


Conclusion The main drug-induced constituents in SCD of SC were dibenzocyclooctadienes and its metabolites.