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Quantitative Determination of Lignan Constituents from Schisandra chinensis extract by Liquid Chromatography

Date:2016/9/19 21:02:16

Quantitative Determination of Lignan Constituents from Schisandra chinensis extract by Liquid Chromatography

The fruits of Schisandra chinensis extract (Schisandraceae/Magnoliaceae) are a traditional oriental medicine 

possessing diverse biological activities. A simple and specific analytical method for the quantitative determination 

of eight lignan constituents from the methanolic extract of the fruits of Schisandra chinensis extract was developed. 


The lignan constituents present in the fruits of Schisandra chinensis were separated with an acetonitrile-water-reagent 

alcohol gradient at a flow rate of 1.0 mL per minute. The HPLC separation was performed on a Phenomenex Luna C18 

(2) (150 × 4.6 mm I.D., particle size 5 μm) reversed phase column with detection at 215 nm. The limit of detection 

was in the range from 0.2 to1.5 μg mL 611 . The relative standard deviation (RSD) values for the determination 

of lignan constituents in fruits of Schisandra chinensis extract were less than 2.0%. 


The method was successfully used to analyze different products available in the market containing Schisandra 

chinensis and also to study the percentage compositions of various lignans present in Schisandra chinensis 

procured from different regions in S. Korea.