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Simultaneous Determination of the Four Lignans in Schisandra chinensis extract and S.sphenanthera by TLCS

Date:2016/9/19 21:03:05

Simultaneous Determination of the Four Lignans in Schisandra chinensis extract and S.sphenanthera by TLCS

To establish a method for determining the content of the four lignans in  Schisandra chinensis extract 

and S.sphenanthera simultaneously. MethodSamples were extracted with alcohol and CO2-SFE. The contents of 

schisandrin,Wuweizi ester A,schisandrin A and schisandrin B could be determined simultaneously by dual 

wavelength TLC- Scanning (λS=220nm,λR=340nm) after the extracts were developed by n-hexane- ethyl acetate 

(8:3) on self-made GF254 plates. 


ResultThe calibration curves were linear in range of 0.44~1.54μg for schisandrin(R=0.9986),0.60~2.10μg 

for Wuweizi ester A(R=0.9960),0.52~1.82μg for schisandrin A(R=0.9997) and 0.48 ~1.68μg for schisandrin 

B(R=0.9962) respectively. The average recoveries(n=5) were 104.5% for schisandrin(RSD=4.0%),95.0% for wuweizi 

ester A(RSD=2.8%),96.1% for schisandrin A (RSD=2.6%) and 102.1% for schisandrin B(RSD=1.9%). All of the 

contents of four lignans in extracts of schisandra with SCF- CO2 are higher than that in  Schisandra 

chinensis extract with alcohol. 


Conclusion: This method was found to be simple,fast and accurate for simultaneously determining the content 

of the four lignans in  Schisandra chinensis extract and S. sphenanthera.