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Study on Supercritical Carbon Dioxide Extract from Schisandra chinensis extract by Gas Chromatography-Mass Spectrometry.

Date:2016/9/13 23:19:01

Study on Supercritical Carbon Dioxide Extract from Schisandra chinensis extract by Gas Chromatography-Mass 

Spectrometry. A portion of 2.2 kg of powdered Schisandra chinensis extract (Turcz.) Baill. was extracted by 

supercritical CO2 under 25 MPa and 45 ℃ for 2 h, and 0319 kg of the extract was obtained. 

The extract was analyzed by gas chromatographymass spectrometry. Experiments were performed on an Agilent 6890N 

Gas Chromatograph with an Agilent 5973N Mass Selective Detector. GC separation was carried out on an HP5MS 

quastz capillary column (25 m×022 mm id×025 μm) at an initial temperature of 150 ℃, hold for 5 min, then 

program at 4 ℃/min until 250 ℃, hold for 10 min. The split ratio was 50∶1. The injector temperature was 

280 ℃ and the injection volume was 1 μL. Mass spectra were collected in the scan range of m/z 30-550. 

All measurements were performed with EI source, electron energy was 70 eV and electron multiplier voltage 

was 12 kV. Solvent delay of 3 min was introduced to the separation. In the extract 16 components were separated,

among which 7 kinds of lignans were identified. The main lignans in the extract were deoxyschizandrin (939%), 

schizandrin B (3058%), schizandrin (1600%), schizandrin C (595%), schizandrol B (1517%).