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Dual effects of extract of Schisandra chinensis extract Baill on rat hepatic CYP3A

Date:2016/9/13 23:15:44

Dual effects of extract of Schisandra chinensis extract Baill on rat hepatic CYP3A. This study is to investigate 

the effects of aqueous extract of Baill (WWZ), kadsurin, schisandrin A, schisandrin B and schisandrol B on hepatic 

CYP3A. received a daily gavage of aqueous extract of WWZ for different times. The livers were harvested after 

gavage and subjected to preparation. Microsomal CYP3A activity was determined by measuring the amount of the 

metabolite of (6 beta-hydroxytestosterone) with HPLC. Aqueous extract of WWZ, kadsurin and schisandrin A were 

incubated with obtained from . Microsomal CYP3A activity was determined by HPLC. Primary hepatocytes were 

separated and extracted from , then were treated with aqueous extract of WWZ, schisandrin A, schisandrin B 

and schisandrol B. Then, the expression of CYP3A1 was analyzed by RT-. As for the in vivo assay, aqueous 

extract of WWZ significantly inhibited the enzyme activity of CYP3A after 12 h gavage. 


The inhibitory effect was converted to inductive effect after 3-day gavage. Aqueous extract of WWZ could induce 

the enzyme activity of CYP3A after 6-day gavage. Aqueous extract of WWZ and kadsurin showed a dose-dependent 

inhibition of CYP3A (IC50 of 487.8 microg mL(-1) and 6.2 micromol L(-1), separately). In primary hepatocytes, 

aqueous extract of WWZ (2.5 mg mL(-1)), schisandrin A (0.1 micromol L(-1)), schisandrin B (0.1 micromol L(-1)) 

and schisandrol B (10 micromol L(-1)) increased significantly the expression of CYP3A1 by 23%, 55%, 42% and 27%, 

respectively. Aqueous extract of WWZ could show dual effect on the enzyme activity of CYP3A in in vivo. 


Meanwhile, kadsurin showed a dose-dependent inhibition of the enzyme activity of hepatic CYP3A in vitro. 

And schisandrin A, schisandrin B and schisandrol B showed significant inductive effect on the expression of 

CYP3A1 .